Application

Frozen Sections (1:300-1:500)Immunoblotting (1:500-1:1000)Free Floating Sections (see application references)Paraffin Sections (see application references; heat pre-treatment required)

General description

Recognizes the ~53 kDa serotonin 2A (5-HT_2A) receptor.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT_2A) receptor.

This Anti-Serotonin 2A Receptor Rabbit pAb is validated for use in Frozen Sections, WB, Free Floating Sections, Paraffin Sections for the detection of Serotonin 2A Receptor.

Immunogen

a synthetic peptide corresponding to amino acids 22-41 of rat 5-HT_2A receptor

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Leonard, B.E. 1996. Psychother. Psychosom.65, 66.Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol.8, 175.

Antibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Glass bottle

Physical form

In 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)